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mouse pparg2  (Addgene inc)


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    Structured Review

    Addgene inc mouse pparg2
    Cos-7 cells endogenously express PPARγ1 (A) and RXRs (B), proteins necessary for PPARγ to activate transcription. Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with ( a ) mouse Pparg1 , mouse <t>Pparg2</t> , human PPARG1 expression vectors , or ( b ) human RXRA, mouse Rxra, mouse Rxrb, mouse Rxrg expression vectors . Lysates were analyzed for PPARγ, RXRα, and βactin expression by immunoblotting
    Mouse Pparg2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse pparg2/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    mouse pparg2 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum"

    Article Title: Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum

    Journal: Environmental Health

    doi: 10.1186/s12940-019-0486-2

    Cos-7 cells endogenously express PPARγ1 (A) and RXRs (B), proteins necessary for PPARγ to activate transcription. Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with ( a ) mouse Pparg1 , mouse Pparg2 , human PPARG1 expression vectors , or ( b ) human RXRA, mouse Rxra, mouse Rxrb, mouse Rxrg expression vectors . Lysates were analyzed for PPARγ, RXRα, and βactin expression by immunoblotting
    Figure Legend Snippet: Cos-7 cells endogenously express PPARγ1 (A) and RXRs (B), proteins necessary for PPARγ to activate transcription. Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with ( a ) mouse Pparg1 , mouse Pparg2 , human PPARG1 expression vectors , or ( b ) human RXRA, mouse Rxra, mouse Rxrb, mouse Rxrg expression vectors . Lysates were analyzed for PPARγ, RXRα, and βactin expression by immunoblotting

    Techniques Used: Transfection, Expressing, Western Blot

    Overexpression of PPARγ significantly increases transcriptional response to rosiglitazone. Cos-7 cells were transfected with reporter plasmids (PPRE-luciferase reporter, CMV-GFP reporter) and empty pcDNA 3.1 or a mouse Pparg2 expression vector. Cells then were treated with Vh (0.5% DMSO) or rosiglitazone (as indicated). Luminescence and fluorescence were measured after 24 h. Data are reported as mean ± standard error ( N = 7 independent transfections). Statistical analyses indicated in the box are from a 2-Factor ANOVA. ** Significantly different from Vh ( p < 0.01, ANOVA, Dunnett’s)
    Figure Legend Snippet: Overexpression of PPARγ significantly increases transcriptional response to rosiglitazone. Cos-7 cells were transfected with reporter plasmids (PPRE-luciferase reporter, CMV-GFP reporter) and empty pcDNA 3.1 or a mouse Pparg2 expression vector. Cells then were treated with Vh (0.5% DMSO) or rosiglitazone (as indicated). Luminescence and fluorescence were measured after 24 h. Data are reported as mean ± standard error ( N = 7 independent transfections). Statistical analyses indicated in the box are from a 2-Factor ANOVA. ** Significantly different from Vh ( p < 0.01, ANOVA, Dunnett’s)

    Techniques Used: Over Expression, Transfection, Luciferase, Expressing, Plasmid Preparation, Fluorescence



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    Image Search Results


    Cos-7 cells endogenously express PPARγ1 (A) and RXRs (B), proteins necessary for PPARγ to activate transcription. Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with ( a ) mouse Pparg1 , mouse Pparg2 , human PPARG1 expression vectors , or ( b ) human RXRA, mouse Rxra, mouse Rxrb, mouse Rxrg expression vectors . Lysates were analyzed for PPARγ, RXRα, and βactin expression by immunoblotting

    Journal: Environmental Health

    Article Title: Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum

    doi: 10.1186/s12940-019-0486-2

    Figure Lengend Snippet: Cos-7 cells endogenously express PPARγ1 (A) and RXRs (B), proteins necessary for PPARγ to activate transcription. Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with ( a ) mouse Pparg1 , mouse Pparg2 , human PPARG1 expression vectors , or ( b ) human RXRA, mouse Rxra, mouse Rxrb, mouse Rxrg expression vectors . Lysates were analyzed for PPARγ, RXRα, and βactin expression by immunoblotting

    Article Snippet: Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with human PPARG1 , human RXRA, mouse Pparg1 (plasmid 8886; Addgene), mouse Pparg2 (plasmid 8865; Addgene) [ ], mouse Rxra, mouse Rxrb, or mouse Rxrg (provided by R. M. Evans, Salk Institute for Biological Studies) [ ].

    Techniques: Transfection, Expressing, Western Blot

    Overexpression of PPARγ significantly increases transcriptional response to rosiglitazone. Cos-7 cells were transfected with reporter plasmids (PPRE-luciferase reporter, CMV-GFP reporter) and empty pcDNA 3.1 or a mouse Pparg2 expression vector. Cells then were treated with Vh (0.5% DMSO) or rosiglitazone (as indicated). Luminescence and fluorescence were measured after 24 h. Data are reported as mean ± standard error ( N = 7 independent transfections). Statistical analyses indicated in the box are from a 2-Factor ANOVA. ** Significantly different from Vh ( p < 0.01, ANOVA, Dunnett’s)

    Journal: Environmental Health

    Article Title: Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum

    doi: 10.1186/s12940-019-0486-2

    Figure Lengend Snippet: Overexpression of PPARγ significantly increases transcriptional response to rosiglitazone. Cos-7 cells were transfected with reporter plasmids (PPRE-luciferase reporter, CMV-GFP reporter) and empty pcDNA 3.1 or a mouse Pparg2 expression vector. Cells then were treated with Vh (0.5% DMSO) or rosiglitazone (as indicated). Luminescence and fluorescence were measured after 24 h. Data are reported as mean ± standard error ( N = 7 independent transfections). Statistical analyses indicated in the box are from a 2-Factor ANOVA. ** Significantly different from Vh ( p < 0.01, ANOVA, Dunnett’s)

    Article Snippet: Whole cell lysates were prepared from Cos-7 cells and Cos-7 cells transfected with human PPARG1 , human RXRA, mouse Pparg1 (plasmid 8886; Addgene), mouse Pparg2 (plasmid 8865; Addgene) [ ], mouse Rxra, mouse Rxrb, or mouse Rxrg (provided by R. M. Evans, Salk Institute for Biological Studies) [ ].

    Techniques: Over Expression, Transfection, Luciferase, Expressing, Plasmid Preparation, Fluorescence